有哪些信誉好的足球投注网站widespread mrna association with cytoskeletal motor proteins and identification and dynamics of myosin-associated mrnas in s. cerevisiae广泛的信使rna与细胞骨架的马达蛋白和识别和动态myosin-associated mrna在酿酒酵母.pdfVIP
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widespread mrna association with cytoskeletal motor proteins and identification and dynamics of myosin-associated mrnas in s. cerevisiae广泛的信使rna与细胞骨架的马达蛋白和识别和动态myosin-associated mrna在酿酒酵母
Widespread mRNA Association with Cytoskeletal Motor Proteins and Identification and Dynamics of Myosin- Associated mRNAs in S. cerevisiae 1,2 3 1,2,4 1,2 3 Jason M. Casolari , Michael A. Thompson , Julia Salzman , Lowry M. Champion , W. E. Moerner , Patrick O. Brown1,2* 1 Department of Biochemistry, Stanford University School of Medicine, Stanford, California, United States of America, 2 Howard Hughes Medical Institute, Stanford University School of Medicine, Stanford, California, United States of America, 3 Department of Chemistry, Stanford University, Stanford, California, United States of America, 4 Department of Statistics, Stanford University, Stanford, California, United States of America Abstract Programmed mRNA localization to specific subcellular compartments for localized translation is a fundamental mechanism of post-transcriptional regulation that affects many, and possibly all, mRNAs in eukaryotes. We describe her e a systematic approach to identify the RNA cargoes associated with the cytoskeletal motor proteins of Saccharomyces cerevisiae in combination with live-cell 3D super-localization microscopy of endogenously tagged mRNAs. Our analysis identified widespread association of mRNAs with cytoskeletal motor proteins, including association of Myo3 with mRNAs encoding key regulators of actin branching and endocytosis such as WASP and WIP. Using conventional fluorescence microscopy and expression of MS2-tagged mRNAs from endogenous loci, we observed a strong bias for actin patch nucleator mRNAs to localize to the cell cortex and the actin patch in a Myo3- and F-actin dependent man
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