wood utilization is dependent on catalase activities in the filamentous fungus podospora anserina木材利用率取决于过氧化氢酶活动的丝状菌柄孢壳菌属anserina.pdfVIP

Wood Utilization Is Dependent on Catalase Activities in the Filamentous Fungus Podospora anserina Anne Bourdais1,2,3,4, Frederique Bidard 1,2, Denise Zickler1,2, Veronique Berteaux-Lecellier1,2,5, 1,2,6 1 Philippe Silar , Eric Espagne * ´ ´ ´ ´ ´ 1 Institut de Genetique et Microbiologie, Univ Paris-Sud, UMR 8621, Orsay, France, 2 CNRS, Orsay, France, 3 Institut Genetique et Developpement de Rennes, CNRS, UMR ´ ´ ´ 6061, Rennes, France, 4 UEB Universite Rennes 1, IFR 140, Faculte de Medecine, Rennes, France, 5 Laboratoire d’Excellence « CORAIL », USR 3278 CNRS-EPHE, CRIOBE, BP ´ 1013, Moorea, French Polynesia, 6 Univ Paris Diderot, Sorbonne Paris Cite, UFR des Sciences du Vivant, Paris, France Abstract Catalases are enzymes that play critical roles in protecting cells against the toxic effects of hydrogen peroxide. They are implicated in various physiological and pathological conditions but some of their functions remain unclear. In order to decipher the role(s) of catalases during the life cycle of Podospora anserina, we analyzed the role of the four monofunctional catalases and one bifunctional catalase-peroxidase genes present in its genome. The five genes were deleted and the phenotypes of each single and all multiple mutants were investigated. Intriguingly, although the genes are differently expressed during the life cycle, catalase activity is dispensable during both vegetative growth and sexual reproduction in laboratory conditions. Catalases are also not essential for cellulose or fatty acid assimilation. In contrast,