wolbachia interferes with ferritin expression and iron metabolism in insects沃尔巴克氏体属干扰铁蛋白表达和铁代谢的昆虫.pdfVIP

Wolbachia Interferes with Ferritin Expression and Iron Metabolism in Insects 1 2 1 2 3 Natacha Kremer *, Denis Voronin , Delphine Charif , Patrick Mavingui , Bertrand Mollereau , Fabrice Vavre1 ´ ´ ´ ´ ´ 1 Universite de Lyon, Lyon; Universite Lyon 1; CNRS, UMR 5558, Laboratoire de Biometrie et Biologie Evolutive, Villeurbanne, France, 2 Universite de Lyon, Lyon; Universite ´ Lyon 1; CNRS, UMR 5557, Laboratoire d’Ecologie Microbienne, Villeurbanne, France, 3 LBMC, UMR5239 CNRS/Ecole Normale Superieure de Lyon, IFR 128 Biosciences Lyon ´ Gerland, Universite de Lyon, Lyon, France Abstract Wolbachia is an intracellular bacterium generally described as being a facultative reproductive parasite. However, Wolbachia is necessary for oogenesis completion in the wasp Asobara tabida. This dependence has evolved recently as a result of interference with apoptosis during oogenesis. Through comparative transcriptomics between symbiotic and aposymbiotic individuals, we observed a differential expression of ferritin, which forms a complex involved in iron storage. Iron is an essential element that is in limited supply in the cell. However, it is also a highly toxic precursor of Reactive Oxygen Species (ROS). Ferritin has also been shown to play a key role in host–pathogen interactions. Measuring ferritin by quantitative RT- PCR, we confirmed that ferritin was upregulated in aposymbiotic compared to symbiotic individuals. Manipulating