建立近交系大鼠肝移植急性排斥模型体会.doc

建立近交系大鼠肝移植急性排斥模型体会 　【关键词】 大鼠,近交系；肝移植；疾病模型,动物；移植物排斥 　　Establishment of acute rejection model of orthotopic liver transplantation in inbred rats 　　【Abstract】 AIM: To establish the orthotopic liver transplantation models in inbred rats and judge the grades of acute rejection. METHODS: Surgical skills were trained by using closed colony SD and Wistar rats, followed by DA to LEW orthotopic liver transplantation with Kamadas twocuff technique. The grades of acute rejection were judged by their clinical manifestations and Banff schema. RESULTS: Fifteen models of DA to LEW liver transplantation were established, the success rate being 86.7%. The causes of death were hemorrhage and thrombosis of suprahepatic and infrahepatic vena cava and obstruction of biliary tract. Ⅰgrade rejection occurred at 3rd day and reached the peak at 7th day after transplantation. Remaining models died of Ⅲ grade rejection besides of postoperative complication within 12 d after transplantation. CONCLUSION: The acute rejection of DA to LEW orthotopic liver transplantation model is stable and intensive. It can be effectively used to study allograft rejection and tolerance. But it is difficult to establish the kind of orthotopic liver transplantation models in inbred rats because of their own tissue characteristics. 　　【Keywords】 rats, inbred strains; liver transplantation; disease models, animal; graft rejection 　　【摘要】目的： 探讨近交系大鼠原位肝移植模型的建立并判断排斥反应发生的强度. 方法： 采用Kamada二袖套法，利用封闭群大鼠SD和Wistar进行建模技能训练，在此基础上建立近交系大鼠DA→LEW肝移植模型，根据临床表现和Banff标准判断排斥反应发生的强度. 结果： 共施行DA→LEW大鼠肝移植模型15例，手术成功率86.7%，死亡原因为肝上下腔静脉漏血、肝下下腔静脉血栓、胆道梗阻. 术后第3天开始出现Ⅰ级排斥反应，7 d以后逐渐达到高峰，除术后并发症致死外，剩余均在12 d内死于Ⅲ级排斥反应. 结论： DA→LEW为稳定、强烈的大鼠肝移植急排模型，是研究肝移植排斥及免疫耐受的理想动物模型. 但近交系大鼠在组织结构上有其自身特点，给建模带来一定难度. 　　【关键词】 大鼠，近交系；肝移植；疾病模型，动物；移植物排斥 　　0引言 　　肝移植动物模型是探讨移植后各种免疫反应发生机制的前提. 缺乏可靠的供受体遗传背景资料的移植免疫研究其价值为零［1］. 近交系大鼠具有遗传背景明确、单一以及生物学特性资料详细等优点，实验时重复性好，研究结果具有可比性，因此对移植免疫学的研究具有重要价值. 本实验利用我校动物实验中心提供的Wistar和SpragueDawley（SD）大鼠，采用Kamada两袖套法进行建模的基本技能训练，在此基础上选择近交系大鼠Dark Agouti（DA）为供体