创伤弧菌脓毒症大鼠肺组织脂多糖受体CD14 mRNA、核因子-κB基.docVIP

创伤弧菌脓毒症大鼠肺组织脂多糖受体CD14 mRNA、核因子-κB基 　 作者：郑阿迈，陈玉熹，李萌芳，梁欢，卢中秋 【摘要】 目的:探讨创伤弧菌（Vibrio vulnificus，VV）脓毒症大鼠肺组织脂多糖受体CD14 mRNA、核因子-κB（NF-κB）基因的表达及其动态变化。方法:SD雄性大鼠60只，随机分为6组，每组10只。第1组：正常对照组（NC组）；第2～6组为VV组: 予大鼠下肢皮下注射VV，造成VV脓毒症模型，以注射VV后2、6、9、12、16 h各时间点分别为2～6组。取各组大鼠部分肺组织，用RT-PCR法测定大鼠肺组织不同时间点CD14 mRNA、NF-κB mRNA的表达。结果: NC组大鼠肺组织有少量CD14、NF-κB mRNA表达，分别为0.392±0.056和0.366±0.179。感染VV后大鼠肺组织CD14 mRNA表达增加，2、6、9、12、16 h的表达量分别为0.976±0.180，1.148±0.218，1.026±0.033，0.988±0.129，0.970±0.125。大鼠肺组织NF-κB mRNA表达量也明显增加，2、6、9、12、16 h的表达量分别为0.763±0.215，1.100±0.101，1.068±0.174，1.057±0.076和0.800±0.205。与NC组相比，二者均明显升高（P＜0.05）。结论:VV脓毒症大鼠肺组织CD14、NF-κB的基因表达水平在脓毒症中明显升高，其与脓毒症的发生、发展密切相关，动态监测其改变对VV脓毒症发病过程具有一定的预警意义。 【关键词】 创伤弧菌；脓毒症；脂多糖受体CD14；核因子-κB；基因 Abstract: Objective: To detect the Gene expression and the dynamic change of pulmonary lipopolysaccharide receptor CD14 and NF-κB in rats with Vibrio vulnificus (VV) sepsis. Methods: Sixty male Sprague-Dawley (SD) rats were equally divided into six groups randomly. The first group was named control group. VV groups include the second to sixth group which were injected VV to make VV sepsis model. Samples were taken from control group and the second group to the sixth group at the timepoint of 2，6，9，12，16 h after injection respectively，the genes of pulmonary lipopolysaccharide receptor CD14 and NF-κB in rats’lung were measured in the different time by the method of RT-PCR. Results: The Gene expression of pulmonary lipopolysaccharide receptor CD14 and NF-κB in the control group was a low level（0.392±0.056，0.366±0.179），but they increased significantly at 2 h（0.976±0.180，0.763±0.215），6 h（1.148±0.218，1.100±0.101），9 h（1.026±0.033，1.068±0.174），12 h（0.988±0.129，1.057±0.076），16 h（0.970±0.125，0.800±0.205）after VV being injected（P ＜0.05）. Conclusion: Our data suggest that the Gene expression of pulmonary lipopolysaccharide receptor CD14 and NF-κB elevation are associated with the development of VV sepsis. Monitoring the change of the Gene expression of pulmonary lipopol