七氟醚迟发性预处理对大鼠局灶性脑缺血损伤后NF-κB表达的影响.docVIP

七氟醚迟发性预处理对大鼠局灶性脑缺血损伤后NF-κB表达的影响 叶治 郭曲练 王锷 （中南大学湘雅医院麻醉科，长沙 410078） [摘要] 目的: 比较不同浓度七氟醚诱导的迟发性脑保护效应以及对大鼠局灶性脑缺血再灌注损伤后不同时点NF-κB蛋白表达的影响。方法: 健康SD雄性大鼠60只，体重220～300 g，随机分为3组：缺血再灌注组（IR组），2.5%七氟醚组（Sevo1组），4.0%七氟醚组（Sevo2组）。采用大鼠大脑中动脉闭塞 (middle cerebral artery occlusion , MCAO) 制备局灶性脑缺血再灌注模型，3组分别于脑缺血前24 h吸入纯氧及2.5% 或4.0%七氟醚+氧气混合气体60min。缺血2 h，再灌注24，72 h后取脑组织，运用TTC染色测算脑梗死体积百分比，免疫组化染色检测NF-κB蛋白表达，从而分析七氟醚的脑保护效应。结果: 与IR组相比，Sevo1组和Sevo2组再灌注24，72 h脑梗死体积显著减少（P0.05）；IR组脑组织顶叶皮层缺血半影区NF-κB表达明显增加，七氟醚能显著抑制脑缺血再灌注后24，72 h缺血半影区NF-κB表达的增加（P0.05）结论:七氟醚对大鼠局灶性脑缺血再灌注损伤具有迟发性保护作用，其机制可能与降低NF-κB蛋白表达有关。 [关键词] 七氟醚；迟发性脑保护作用；局灶性脑缺血再灌注损伤；NF-κB； The delayed neuroprotective effect of sevoflurane on expression of NF-κB following transient focal ischemia-reperfusion in rats. YE Zhi, GUO Qu-lian , WANG E, （Department of Anesthesia , Xiangya Hospital , Changsha , 410078 , China） Abstract：Objective To investigate the delayed neuroprotective effect of sevoflurane on expression of NF-κB in rat models of transient focal ischemia-reperfusion. Methods Adult male Sprague-Dawley rats (220～300g) were randomly assigned into three groups: Ischemia-reperfusion (I/R), 2.5% Sevoflurane (Sevo1), 4.0% Sevoflurane (Sevo2) groups, and subjected to right middle cerebral artery occlusion (MCAO) for two hours. Sevoflurane preconditioning was induced 24 h before brain ischemia by exposing the animals to 2.5% or 4.0% sevoflurane + oxygen for 60 min. The animals in the IR group were exposed to 100% oxygen 60 min at 24 h before MCAO. We analyzed the effeets of sevoflurane on brain by evaluating the infarct volume and the expressions of NF-κB through TTC staining and immunohistochemistry at 24 and 72 h after reperfusion, respectively. Results Sevoflurane could obviously reduced the brain damage after ischemia-reperfusion，and the infarct volumes were significantly reduced in the Sevo1 and Sevo2 group ，compared with the IR group. The expressions of NF-κB in ischemic territory increased after cerebral isehemia, sevoflurane could decrease the