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antioxidant and cytotoxic activities of centella asiatica (l) urb.抗氧化和细胞毒性积雪草的活动(l)市区。
Int. J. Mol. Sci. 2009, 10, 3713-3721; doi:10.3390/ijm
OPEN ACCESS
International Journal of
Molecular Sciences
ISSN 1422-0067
/journal/ijms
Article
Antioxidant and Cytotoxic Activities of Centella asiatica (L)
Urb.
Frederico Pittella 1, Rafael C. Dutra 1, Dalton D. Junior 2, Miriam T. P. Lopes 2 and Nádia R.
Barbosa 1,*
1 Departamento de Alimentos e Toxicologia, Faculdade de Farmácia e Bioquímica, Universidade
Federal de Juiz de Fora, Martelos, CEP 36036-330, Juiz de Fora, MG, Brazil;
E-Mail: fredpittella@ (F.P.)
2 Laboratório de Substâncias Antitumorais, Instituto de Ciências Biológicas, Universidade Federal de
Minas Gerais, CEP 31270-901, Belo Horizonte, MG, Brazil;
E-Mail: mtpl@icb.ufmg.br (M.T.P.L.)
* Author to whom correspondence should be addressed; E-Mail: nadiafox@.
Received: 26 July 2009 / Accepted: 26 August 2009 / Published: 26 August 2009
Abstract: In the present study, the phenolic (Folin-Dennis) and flavonoid (colorimetric
assay) constituents, antioxidant [2,2-diphenyl-2-picrylhydrazyl hydrate (DPPH) assay] and
cytotoxic activities of an aqueous extract (AE) of Centella asiatica leaves were
investigated. The aqueous extract (50 g/L) was obtained by infusion followed by cold
maceration for 24 h. The levels of phenolic and flavonoid compounds were 2.86 g/100 g
and 0.361 g/100 g, respectively. The AE showed elevated DPPH scavenging activity, with
an
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