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antioxidant activities of fractions of polymeric procyanidins from stem bark of acacia confusa分数的抗氧化活动聚合原花青素来自金合欢confusa干树皮
Int. J. Mol. Sci. 2011, 12, 1146-1160; doi:10.3390/ijm
OPEN ACCESS
International Journal of
Molecular Sciences
ISSN 1422-0067
/journal/ijms
Article
Antioxidant Activities of Fractions of Polymeric Procyanidins
from Stem Bark of Acacia confusa
Shu-Dong Wei, Hai-Chao Zhou and Yi-Ming Lin *
Key Laboratory of the Ministry of Education for Coastal and Wetland Ecosystems, School of Life
Sciences, Xiamen University, Xiamen 361005, China; E-Mails: weishudong2005@126.com (S.-D.W.);
seapass2004@163.com (H.-C.Z.)
* Author to whom correspondence should be addressed; E-Mail: linym1967@;
Tel.: +86-592-2187657.
Received: 13 January 2011; in revised form: 30 January 2011 / Accepted: 7 February 2011 /
Published: 15 February 2011
Abstract: The polymeric procyanidins extracted from Acacia confusa stem bark were
fractionated with a step gradient of water, methanol and acetone on a Sephadex LH-20
column. The antioxidant activity of the collected fractions was investigated by the
2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging and ferric
reducing/antioxidant power (FRAP) assays. All fractions possessed potent antioxidant
activity with the highest activity observed for fraction F9. The matrix-assisted laser
desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) and
reversed-phase high
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