algae-produced pfs25 elicits antibodies that inhibit malaria transmissionalgae-produced pfs25抒发抗体抑制疟疾传播.pdfVIP

Algae-Produced Pfs25 Elicits Antibodies That Inhibit Malaria Transmission 1 2 1 1 1 2 James A. Gregory , Fengwu Li , Lauren M. Tomosada , Chesa J. Cox , Aaron B. Topol , Joseph M. Vinetz , Stephen Mayfield1* 1 Division of Biological Sciences, and the San Diego Center for Algae Biotechnology, University of California San Diego, California, United States of America, 2 Division of Infectious Disease, Department of Medicine, University of California San Diego, La Jolla, California, United States of America Abstract Subunit vaccines are significantly more expensive to produce than traditional vaccines because they are based primarily on recombinant proteins that must be purified from the expression system. Despite the increased cost, subunit vaccines are being developed because they are safe, effective, and can elicit antibodies that confer protection against diseases that are not currently vaccine-preventable. Algae are an attractive platform for producing subunit vaccines because they are relatively inexpensive to grow, genetically tractable, easily scaled to large volumes, have a short generation time, and are devoid of inflammatory, viral, or prion contaminants often present in other systems. We tested whether algal chloroplasts can produce malaria transmission blocking vaccine candidates, Plasmodium falciparum surface protein 25 (Pfs25) and 28 (Pfs28). Antibodies that recognize Pfs25 and Pfs28 disrupt the sexual development of parasites within the mosquito midgut, thus preventing transmission of malaria from one human host to the next. These proteins have been difficult to produce in traditional recombinant systems because they contain tandem repeats of structurally complex epidermal growth factor-like domains, which can