additive effect of rpb27 immunization and chemotherapy in experimental paracoccidioidomycosis添加剂在实验paracoccidioidomycosis rpb27免疫和化疗的效果.pdfVIP

Additive Effect of rPb27 Immunization and Chemotherapy in Experimental Paracoccidioidomycosis 1 ˆ 2 3 1 ´ Viviane C. Fernandes *, Estefania M. N. Martins , Jankerle N. Boeloni , Juliana B. Coitinho , Rogeria Serakides3, Alfredo M. Goes1 ´ ˆ ´ 1 Departamento de Bioquımica e Imunologia, Instituto de Ciencias Biologicas, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil, 2 Departamento de ˆ ´ ´ ´ Microbiologia, Instituto de Ciencias Biologicas, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil, 3 Departamento de Clınica e Cirurgia Veterinaria, Escola de ´ Veterinaria da Universidade Federal de Minas Gerais, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil Abstract Paracoccidioidomycosis, PCM, the major systemic mycosis in Latin America, is caused by the termally dimorphic fungus Paracoccidioides brasiliensis and requires extended periods of chemotherapy with a significant frequency of relapsing disease. The search for new alternatives of treatment is necessary. rPb27 is an antigenic protein from P. brasiliensis that already showed a significant protective activity as a vaccine for PCM in experimental models. The cDNA of rPb27 was subcloned into a pET-DEST 42 plasmid, expressed in E. coli with a his-tag and purified by affinity chromatography. Immunization with this recombinant protein and chemotherapy were used together in an attempt to improve treatment of PCM. For this,