adaptation to er stress is mediated by differential stabilities of pro-survival and pro-apoptotic mrnas and proteins适应er压力是由微分pro-survival pro-apoptotic mrna和蛋白的稳定性.pdfVIP

PLoS BIOLOGY Adaptation to ER Stress Is Mediated by Differential Stabilities of Pro-Survival and Pro-Apoptotic mRNAs and Proteins 1 2¤ 2 2 2 2 3 D. Thomas Rutkowski , Stacey M. Arnold , Corey N. Miller , Jun Wu , Jack Li , Kathryn M. Gunnison , Kazutoshi Mori , Amir A. Sadighi Akha4 5 1,2,6* , David Raden , Randal J. Kaufman 1 Howard Hughes Medical Institute, University of Michigan Medical Center, Ann Arbor, Michigan, United States of America, 2 Department of Biological Chemistry, University of Michigan Medical Center, Ann Arbor, Michigan, United States of America, 3 Department of Biophysics, Graduate School of Science, Kyoto University, Kyoto, Japan, 4 Department of Pathology, University of Michigan Medical Center, Ann Arbor, Michigan, United States of America, 5 Department of Chemical Engineering, University of Delaware, Newark, Delaware, United States of America, 6 Department of Internal Medicine, University of Michigan Medical Center, Ann Arbor, Michigan, United States of America The accumulation of unfolded proteins in the endoplasmic reticulum (ER) activates a signaling cascade known as the unfolded protein response (UPR). Although activation of the UPR is well described, there is little sense of how the response, which initiates both apoptotic and adaptive pathways, can selectively allow for adaptation. Here we describe the reconstitution of an adaptive ER stress response in a cell culture system. Monitoring the activation an