ablation of dihydroceramide desaturase confers resistance to etoposide-induced apoptosis in vitro消融的dihydroceramide desaturase抵抗etoposide-induced体外细胞凋亡.pdfVIP

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ablation of dihydroceramide desaturase confers resistance to etoposide-induced apoptosis in vitro消融的dihydroceramide desaturase抵抗etoposide-induced体外细胞凋亡.pdf

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ablation of dihydroceramide desaturase confers resistance to etoposide-induced apoptosis in vitro消融的dihydroceramide desaturase抵抗etoposide-induced体外细胞凋亡

Ablation of Dihydroceramide Desaturase Confers Resistance to Etoposide-Induced Apoptosis In Vitro 1 1,2 3 1 1 Monowarul M. Siddique *, Benjamin T. Bikman , Liping Wang , Li Ying , Erin Reinhardt , 4 4 1,3 Guanghou Shui , Markus R. Wenk , Scott A. Summers * 1 Program in Cardiovascular and Metabolic Diseases, Duke-National University of Singapore Graduate Medical School, Singapore, Singapore, 2 Department of Physiology and Developmental Biology, Brigham Young University, Provo, Utah, United States of America, 3 Sarah W. Stedman Nutrition and Metabolism Center, Duke University Medical Center, Durham, North Carolina, United States of America, 4 Department of Biochemistry, National University of Singapore, Singapore, Singapore Abstract Sphingolipid biosynthesis is potently upregulated by factors associated with cellular stress, including numerous chemotherapeutics, inflammatory cytokines, and glucocorticoids. Dihydroceramide desaturase 1 (Des1), the third enzyme in the highly conserved pathway driving sphingolipid biosynthesis, introduces the 4,5-trans-double bond that typifies most higher-order sphingolipids. Surprisingly, recent studies have shown that certain chemotherapeutics and other drugs inhibit Des1, giving rise to a number of sphingolipids that lack the characteristic double bond. In order to assess the effect of an altered sphingolipid profile (via Des1 inhibition) on cell function, we generated isogenic mouse embryonic fibroblasts lacking both Des1 alleles. Lipidomic profiling revealed that these cells contained higher levels of dihydroceramide than wild- type