absence of detectable xmrv and other mlv-related viruses in healthy blood donors in the united states没有检测到xmrv病毒和其他mlv-related健康献血者在美国.pdfVIP

Absence of Detectable XMRV and Other MLV-Related Viruses in Healthy Blood Donors in the United States Shixing Tang*, Jiangqin Zhao, Mohan Kumar Haleyur Giri Setty, Krishnakumar Devadas, Durga Gaddam, Ragupathy Viswanath, Owen Wood, Panhe Zhang, Indira K. Hewlett* Lab of Molecular Virology, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, Maryland, United States of America Abstract Background: Preliminary studies in chronic fatigue syndrome (CFS) patients and XMRV infected animals demonstrated plasma viremia and infection of blood cells with XMRV, indicating the potential risk for transfusion transmission. XMRV and MLV-related virus gene sequences have also been detected in 4–6% of healthy individuals including blood donors in the U.S. These results imply that millions of persons in the U.S. may be carrying the nucleic acid sequences of XMRV and/or MLV- related viruses, which is a serious public health and blood safety concern. Methodology/Principal Findings: To gain evidence of XMRV or MLV-related virus infection in the U.S. blood donors, 110 plasma samples and 71 PBMC samples from blood donors at the NIH blood bank were screened for XMRV and MLV-related virus infection. We employed highly sensitive assays, including nested PCR and real-time PCR, as well as co-culture of plasma with highly sensitive indicator DERSE cells. Using these assays, none of the samples were positive for XMRV or MLV-related virus. Conclusions/Significance: Our results are consistent with those from several other studies, and demonstrate the absence of XMRV or MLV-related viruses in the U.S. blood donors that we studied. Citation: Tang S, Zhao J, Haleyur Giri Setty MK, Devadas K, Gaddam D, et al. (2011) Absence of Detectable XMRV and Other MLV-Related Viruses in Healthy Blood Donors in the United States. PLoS ONE 6(11): e27391. doi:10.1371/journal.pone.0