abnormal production of pro- and anti-inflammatory cytokines by lupus monocytes in response to apoptotic cells生产支持和抗炎细胞因子的异常狼疮单核细胞凋亡细胞的反应.pdfVIP

Abnormal Production of Pro- and Anti-Inflammatory Cytokines by Lupus Monocytes in Response to Apoptotic Cells 1 2,3 2 2 2 Sangeeta Sule , Antony Rosen , Michelle Petri , Ehtisham Akhter , Felipe Andrade * 1 Department of Pediatrics, Johns Hopkins University School of Medicine, Baltimore, Maryland, United States of America, 2 Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland, United States of America, 3 Department of Pathology, Johns Hopkins University School of Medicine, Baltimore, Maryland, United States of America Abstract Monocytes are a key component of the innate immune system involved in the regulation of the adaptive immune response. Previous studies have focused on apoptotic cell clearance abnormalities in systemic lupus erythematosus (SLE) monocytes. However, whether SLE monocytes might express unique patterns of cytokine secretion in response to apoptotic cells is still unknown. Here, we used monocytes from healthy controls and SLE patients to evaluate the production of TNF-a and TGF-b in response to apoptotic cells. Upon recognition of apoptotic material, monocytes from healthy controls showed prominent TGF- b secretion (mean 6 SD: 824.66144.3 pg/ml) and minimal TNF-a production (mean 6 SD: 32.662.1 pg/ml). In contrast, monocytes from SLE patients had prominent TNF-a production (mean 6 SD: 302.26337.5 pg/ml) and diminished TGF-b secretion (mean 6 SD: 685.96615.9 pg/ml), a difference that was statistically significant compared to normal monocytes (p#1026 for TNF-a secretion, and p = 0.0031 for TGF-b, respectively). Interestingly, the unique cytokine response by SLE monocytes was independent of their phagocytic clearance efficiency, opsonizing aut