a stress surveillance system based on calcium and nitric oxide in marine diatoms压力监测系统基于钙和一氧化氮在海洋硅藻.pdfVIP

PLoS BIOLOGY A Stress Surveillance System Based on Calcium and Nitric Oxide in Marine Diatoms 1,2[ 2,3[ 4 1,2 4 4 Assaf Vardi , Fabio Formiggini , Raffaella Casotti , Alessandra De Martino , Franc¸ois Ribalet , Antonio Miralto , Chris Bowler1,2* ´ 1 Laboratory of Diatom Signalling and Morphogenesis, Ecole Normale Superieure, Paris, France, 2 Laboratory of Cell Signalling, Stazione Zoologica Anton Dohrn, Villa Comunale, Naples, Italy, 3 Section of Molecular Cytology Centre for Advanced Microscopy, University of Amsterdam, Kruislaan, Amsterdam, Netherlands, 4 Laboratory of Ecophysiology, Stazione Zoologica Anton Dohrn, Villa Comunale, Naples, Italy Diatoms are an important group of eukaryotic phytoplankton, responsible for about 20% of global primary productivity. Study of the functional role of chemical signaling within phytoplankton assemblages is still in its infancy although recent reports in diatoms suggest the existence of chemical-based defense strategies. Here, we demonstrate how the accurate perception of diatom-derived reactive aldehydes can determine cell fate in diatoms. In particular, the aldehyde (2E,4E/Z)-decadienal (DD) can trigger intracellular calcium transients and the generation of nitric oxide (NO) by a calcium-dependent NO synthase-like activity, which results in cell death. However, pretreatment of cells with sublethal doses of aldehyde can induce resistance to subsequent lethal doses, which is reflected in an altered calcium signature and kinetics of NO production. We als