a phagocytic route for uptake of double-stranded rna in rnai吞噬的路线在rnai的双链rna.pdfVIP

A Phagocytic Route for Uptake of Double-Stranded RNA in RNAi ˜ 1 . 1.¤ 2 1 Joao J. E. Rocha * , Viktor I. Korolchuk , Iain M. Robinson , Cahir J. O’Kane * 1 Department of Genetics, University of Cambridge, Cambridge, United Kingdom, 2 Peninsula College of Medicine and Dentistry, University of Exeter, Plymouth, United Kingdom Abstract RNA interference (RNAi) has a range of physiological functions including as a defence mechanism against viruses. To protect uninfected cells in a multicellular organism, not only a cell-autonomous RNAi response is required but also a systemic one. However, the route of RNA spread in systemic RNAi remains unclear. Here we show that phagocytosis can be a route for double-stranded RNA uptake. Double-stranded RNA expressed in Escherichia coli induces robust RNAi in Drosophila S2 cells, with effectiveness comparable to that of naked dsRNA. We could separate this phagocytic uptake route from that for RNAi induced by naked dsRNA. Therefore, phagocytic uptake of dsRNA offers a potential route for systemic spread of RNAi. Citation: Rocha JJE, Korolchuk VI, Robinson IM, O’Kane CJ (2011) A Phagocytic Route for Uptake of Double-Stranded RNA in RNAi. PLoS ONE 6(4): e19087. doi:10.1371/journal.pone.0019087 Editor: Francois Schweisguth, Institut Pasteur, France Received August 19, 2010; Accepted March 28, 2011; Published April 29, 2011 Copyright: 2011 Rocha et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Funding: This work was funded by grants G0802208 from the Medical Research Council to IMR and 8/C1