a high protein diet during pregnancy affects hepatic gene expression of energy sensing pathways along ontogenesis in a porcine model怀孕期间高蛋白饮食会影响肝基因表达的能量感应途径以及猪模型中的个体发育.pdfVIP

A High Protein Diet during Pregnancy Affects Hepatic Gene Expression of Energy Sensing Pathways along Ontogenesis in a Porcine Model 1 1 2 3 1 Michael Oster , Eduard Murani , Cornelia C. Metges , Siriluck Ponsuksili , Klaus Wimmers * 1 Research Unit Molecular Biology, Leibniz Institute for Farm Animal Biology (FBN), Dummerstorf, Germany, 2 Research Unit Physiology of Nutrition, Leibniz Institute for Farm Animal Biology (FBN), Dummerstorf, Germany, 3 Research Group Functional Genomics, Leibniz Institute for Farm Animal Biology (FBN), Dummerstorf, Germany Abstract In rodent models and in humans the impact of gestational diets on the offspring’s phenotype was shown experimentally and epidemiologically. The underlying programming of fetal development was shown to be associated with an increased risk of degenerative diseases in adulthood, including the metabolic syndrome. There are clues that diet-dependent modifications of the metabolism during fetal life can persist until adulthood. This leads to the hypothesis that the offspring’s transcriptomes show short-term and long-term changes depending on the maternal diet. To this end pregnant German landrace gilts were fed either a high protein diet (HP, 30% CP) or an adequate protein diet (AP, 12% CP) throughout pregnancy. Hepatic transcriptome profiles of the offspring were analyzed at prenatal (94 dpc) and postnatal stages (1, 28, 188 dpn). Depending on the gestational dietary exposure, mRNA expression levels of genes related to energy metabolism, N-metabolism, growth factor signaling pathways, lipid metabolism, nucleic acid metabolism and stress/immune response were affected either in a short-term or in a long-term manner. Gene expression profiles at fetal