湖南地区I型乙型脑炎病毒的分离鉴定及E基因序列分析.pdfVIP

() 2017, 43(2):195–199. DOI:10.13331/ki.jhau.2017.02.015 Journal of Hunan Agricultural University (Natural Sciences) 湖南地区 I 型乙型脑炎病毒的分离鉴定及 E 基因序列分析 * ( 410128) 摘 要(JEV) 3 JEV 3 3 900 RT–PCR JEV NS5 40 17 BHK–21 3 1 JEV HNML1 JEV E 1 RT–PCR HNML1 E E SA14–14–2 86.9% E SA14–14–2 97.0% 15 ( 8 ) HNML1 I JEV 关 键 词I 中图分类号S852.65 文献标志码A 文章编号1007− 1032(2017)02−0195−05 Isolation and identification the Japanese encephalitis virus of genotype I in Hunan province and sequence analysis on its E gene * XIANG Weijun, YANG Taotao, LI Runcheng, YU Xinglong (College of Veterinary Medicine, Hunan Agricultural University, Changsha 410128, China) Abstract: In order to explore Japanese encephalitis virus carried by mosquitoes in farms of Hunan Miluo, a JEV pathogenic investigation on mosquitoes was conducted in three farms of the region. Gene JEV NS5 collected from about 3 900 mosquitoes in 3 pig farms was amplified using RT–PCR. The result showed that out of 17 in total 40 samples was positive. The JEV was isolated from the positive grinding fluid using the BHK–21 cell method. One JEV was obtained after 3 passages, and was named by HNML1 . A pair of primer was designed according to gene JEV E, gene E from HNML1 was amplified, and sequencing and homology analysis were conducted as well. The results showed that the homology on nucleotide sequence and amino acid sequence of gene E to corresponding sequences in live antigen attenuated vaccine strains SA14–14–2 was 86.9% and 97.0%, respectively. There were 15 differences of amino acids, which included 8 key amino acid