体外培养血管平滑肌细胞及内皮细胞增殖影响.docVIP

体外培养血管平滑肌细胞及内皮细胞增殖影响作者单位：第四军医大学西京医院整形外科中心（西安　710032） 摘　要　目的：研究在体外培养条件下兔血管内皮细胞条件培养基(E CCM)、内皮素-1(ET-1)、内皮素转化酶抑制剂(Endothelial-coverting enzyme inhibit or)Phosphoramidon 对血管平滑肌细胞(SMC)增殖的影响，同时研究了血管平滑肌细胞条件 培养基(SMCCM)对血管内皮细胞(EC)增殖方面的影响。方法：EC和SMC均来源于兔主动脉，在 获得了EC和SMC的条件培养基(CM)后，分别用两者以及ET-1进行实验，细胞的增殖率通过? 3H掺入法进行测定。结果：ECCM和ET-1可明显促进SMC的增殖，并呈现剂量依赖性。其最大 效应分别为190%±11%(100% ECCM)和166%±9%(100pg/ml ET-1)。而Phosphoramidon存在条 件下的ECCM使其分裂作用降低33%±2%。SMCCM抑制EC的增殖，这种抑制作用并非剂量依赖性 ，其最大的抑制效应为基本水平的78%±3%。结论：ECCM和ET-1可促进SMC的增殖。Phospho ramidon可明显地抑制ECCM对SMC的增殖作用。同时SMCCM对EC的增殖起抑制作用。 关键词　血管内皮细胞　血管平滑肌细胞　增殖 MODULATIONS OF PROLIFERATION BETWEEN VASCULAR ENDOTHELIAL CELL AND VASCULAR SMOOTH MUSCLE CELL IN VITRO Pan Yong　Ai Yufeng Department of Plastic Surgery, Xijing Hospital,Fourth Military Medical Uni versity(Xian 710032) Ａｂｓｔｒａｃｔ　Aim:To study the effects of vascular end othelial cell conditioned medium(ECCM), endothelin-1 (ET-1), Endothelin-conve rting enzyme inhibitor Phosphoramidon on the proliferation of vascular smooth mu scle cell (SMC) and vascular smooth muscle cell conditioned medium(SMCCM) on the proliferation of vascular endothelial cell(EC)in vitro.Methods:EC and SMC were isolated from rabbit aortas and cultured.The conditioned medium(CM) of EC and SM C were harvested.ECCM,ECCM conditioned in the presence of Phosphoramidon,SMCCM a nd ET-1 were added individually to the cultures.Proliferation rate of the cells was measured with ?3 H-thymidine incorporation on 3 days.　Result:ECCM and E T-1 significantly increased the proliferation of SMC in a dose-dependent manne r with a maximal effect of 199%±11%(100% ECCM) and 166%±9%(100pg/ml ET-1)resp ectively.ECCM conditioned in the presence of Phosphoramidon reduced the mitogeni c effect by 33%±2%.SMC conditioned medium elicited a dose dependent decrease of cultured EC proliferation with a maximal effect of 78%±3% over basal level.　C onclusion:ECCM and ET-1 had a mitogenic effect on SMC.Phosphoramidon could sign ificantly reduce