Chronic myeloid leukemia smac and survivin gene expression and significance of（慢性骨髓性白血病smac和生存素基因表达和意义）.doc

Chronic myeloid leukemia smac and survivin gene expression and significance of Author: WANG Shu-country pipe Hung Hu new flower Gong Guoting [Abstract] Objective To examine the chronic myeloid leukemia (CML) patients smac of survivin mRNA expression, to explore its significance in CML disease progression and prognosis. Methods Semi-quantitative RT PCR detected 71 cases of CML patients and 10 healthy the above gene expression results of smac and survivin mRNA positive expression rate of CML patients were 90.1%, 63.4%, significantly higher than the normal control group (χ 2 = 22.176,6.773, P lt;0.01, 0.05), both in of CML in accelerated phase, blast phase expression level was significantly higher than in chronic phase (t = 10.708 ~ 16.725, P lt;0.01) between the expression level in CML patients was positively correlated (r = 0.833, P lt;0.01)., smac and survivin gene and CML progression is closely related to the monitoring of CML disease progression and prognosis of valuable molecular markers. [Key Words] leukemia, myeloid, chronic, genetic smac gene of survivin in; gene expression [ABSTRACT] ObjectiveTo detect the expressions of smac and survivin mRNA in chronic myeloid leukemia (CML) and explore their clinical significance.MethodsUsing semi quantitative RT PCR technique, expressions of smac and survivin mRNA were detected in 71 CML patients and 10 healthy adults.ResultsA positive rate of smac and survivin in the patients was 90.1% and 63.4%, respectively, significantly higher than that of the controls (χ2 = 22.176,6.773; P lt;0.01,0.05). Both smac and survivin expressions were much higher in accelerated phase and acute transformation phase than in chronic phase (t = 10.708-16.725, P lt;0.01). The expression level of smac and survivin showed positive correlation (r = 0.833, P lt;0.01). ConclusionThe smac and survivin genes are closely related to the advancement and prognosis of CML, which are valuable molecular markers to be used for monitoring the dis