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lE^掌博±掌&*i 英文摘要
ratewasdeterminedMT]1 andcell was
proliferation by assay cyclinganalyzedby
flow and
cytometricmethod.Superoxide lipid
shownaccumulation detected
by by
peroxidation,as
biochemical of levels)and
assay.Expressionsprocollagen1.procollagen】lJ(protein
detectedELISAand
MMP一1,MMP一3(mRNA
level)wereby
reverse
transcriptase—PCRseparately.
Inanimal modelwithchronic
study,the pig
guineaphotoaging
wasset effectsof
radiation(SSR)exposureupfirstly.Theprotective Tempol,
usedbeforeeach attheconcentrationof5 or0.5
topically exposure mg/ml mg/ml,
werealsoassessed.Dermalstructurewasobservedafter stainor
Hematoxylin—eosin
electron andMDAlevelintheskinweredetected
by activity by
microscopy.SOD
biochemical structureandthe ofelasticfiberwere
assay.The expression analyzed
stain structureof 1 and 111wasobservedafter
after andthe
Weigert‘S collagencollagen
Siriured’Sstain
bypolarizedmicroscopy.
Kesults:
lnin—vitro curveafterUVA1 orUVBirradiationshoweddose
study.cellproliferation
decrement cells attheG0/G1 andthe
dependent
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