聚丙烯酰胶凝胶电泳（Polyacrylamide gel electrophoresis）.docVIP

聚丙烯酰胶凝胶电泳（Polyacrylamide gel electrophoresis） (6) 10% ammonium persulfate (AP): ready to use. (7) electrophoresis buffer (Tris- glycine buffer PH8.3): take Tris 6.0g, glycine 28.8g, and dissolve it in 1L without ion water. (8) 1% (W) bromo phenol blue: take 100mg bromo phenol blue, add distilled water 10ml, stir until completely dissolved and filtered. (9) 5X sample buffer (loading, buffer):10ml 3.1ml 1mol/l Tris-HCL (PH6.7) 5ml 50% (V) glycerol 25% 0.5ml 1% (W) bromo phenol blue 0.05% 1.4ml distilled water 4) operation steps 1, installation of the sandwich type vertical slab electrophoresis tank: electrode groove on both sides of the sandwich type vertical plate electrophoresis tank is made of organic glass, a gel die between two electrode groove, the mould is composed of a U-shaped silicone frame, long and short glass plate, template comb, electrophoresis tank by tank (Platinum electrode with short glass under the tank (plate), platinum electrode facing long glass plate) and paper condenser tube composition, two electrode groove and the gel tank mode by screws, the assembly order: Loading tank and fixing screw pin; The long and short glass plate are respectively inserted into the cleaning groove of the U-shaped silicon rubber frame, careful not to touch the glass glue; Insert the gel mold of the inserted glass plate into the storage tank, and the short glass plate shall face the upper storage tank. The long glass plate shall face the storage tank, and both hands will screw the cap in a diagonal manner; The vertical tank, draw 1% agarose solution with a small amount of the dropper, poured into the gel at the bottom of the template (lateral, long glass plate along the concave groove, small) level of about 0.5 ~ 1.0cm, the agarose solidified, which blocked the narrow slit below the gel mold (electricity and can be used as a salt bridge). 2, with glue: according to the measured protein molecular weight range, select the appropriate separation gel concentration. In thi