有哪些信誉好的足球投注网站natural variation in seed very long chain fatty acid content is controlled by a new isoform of kcs18 in arabidopsis thaliana自然变化的种子非常长链脂肪酸的内容是由一个新的kcs18同种型的拟南芥.pdfVIP
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natural variation in seed very long chain fatty acid content is controlled by a new isoform of kcs18 in arabidopsis thaliana自然变化的种子非常长链脂肪酸的内容是由一个新的kcs18同种型的拟南芥
Natural Variation in Seed Very Long Chain Fatty Acid Content Is Controlled by a New Isoform of KCS18 in Arabidopsis thaliana 1,2 ´ 1,2 1,2 1,2 3 Sophie Jasinski *, Alain Lecureuil , Martine Miquel , Olivier Loudet , Sylvain Raffaele , Marine Froissard1,2, Philippe Guerche1,2 1 INRA, UMR1318, Institut Jean-Pierre Bourgin, RD10, Versailles, France, 2 AgroParisTech, Institut Jean-Pierre Bourgin, RD10, Versailles, France, 3 The Sainsbury Laboratory, Norwich Research Park, Norwich, United Kingdom Abstract Oil from oleaginous seeds is mainly composed of triacylglycerols. Very long chain fatty acids (VLCFAs) are major constituents of triacylglycerols in many seed oils and represent valuable feedstock for industrial purposes. To identify genetic factors governing natural variability in VLCFA biosynthesis, a quantitative trait loci (QTL) analysis using a recombinant inbred line population derived from a cross between accessions Bay-0 and Shahdara was performed in Arabidopsis thaliana. Two fatty acid chain length ratio (CLR) QTL were identified, with one major locus, CLR.2, accounting for 77% of the observed phenotypic variation. A fine mapping and candidate gene approach showed that a key enzyme of the fatty acid elongation pathway, the b-ketoacyl-CoA synthase 18 (KCS18), was responsible for the CLR.2 QTL detected between Bay-0 and Shahdara. Association genetics and heterologous expression in yeast cells identified a single point mutation associated with an alteration of KCS18 activity, uncovering the molecular bases for the modulation of VLCFA content in these two natural populations of Arabidopsis. Identification of this kcs18 mutant with
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