muscle-bound primordial stem cells give rise to myofiber-associated myogenic and non-myogenic progenitors肌肉僵硬的原始干细胞产生myofiber-associated non-myogenic祖细胞和肌原性的.pdfVIP

Muscle-Bound Primordial Stem Cells Give Rise to Myofiber-Associated Myogenic and Non-Myogenic Progenitors 1. 2. 3. 1 1 1 Elad Segev , Gabi Shefer , Rivka Adar , Noa Chapal-Ilani , Shalev Itzkovitz , Inna Horovitz , Yitzhak 4 2 1,3 Reizel , Dafna Benayahu , Ehud Shapiro * 1 Department of Computer Science and Applied Mathematics, Weizmann Institute of Science, Rehovot, Israel, 2 Department of Cell and Developmental Biology, Sackler School of Medicine, Tel-Aviv University, Tel-Aviv, Israel, 3 Department of Biological Chemistry, Weizmann Institute of Science, Rehovot, Israel, 4 Department of Biological Regulation, Weizmann Institute of Science, Rehovot, Israel Abstract Myofiber cultures give rise to myogenic as well as to non-myogenic cells. Whether these myofiber-associated non-myogenic cells develop from resident stem cells that possess mesenchymal plasticity or from other stem cells such as mesenchymal stem cells (MSCs) remain unsolved. To address this question, we applied a method for reconstructing cell lineage trees from somatic mutations to MSCs and myogenic and non-myogenic cells from individual myofibers that were cultured at clonal density. Our analyses show that (i) in addition to myogenic progenitors, myofibers also harbor non-myogenic progenitors of a distinct, yet close, lineage; (ii) myofiber-associated non-myogenic and myogenic cells share the same muscle-bound primordial stem cells of a lineage distinct from bone marrow MSCs; (iii) these muscle-bound primordial stem-cells first part to individual muscles and then differentiate into myogenic and non-myogenic stem cells. Citation: Segev E, Shefer G, Adar R, Chapal-Ilani N, Itzkovitz S, et