mrna-seq of single prostate cancer circulating tumor cells reveals recapitulation of gene expression and pathways found in prostate cancermrna-seq单一循环肿瘤细胞显示前列腺癌基因表达的重演和途径中发现前列腺癌.pdfVIP
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mrna-seq of single prostate cancer circulating tumor cells reveals recapitulation of gene expression and pathways found in prostate cancermrna-seq单一循环肿瘤细胞显示前列腺癌基因表达的重演和途径中发现前列腺癌
mRNA-Seq of Single Prostate Cancer Circulating Tumor Cells Reveals Recapitulation of Gene Expression and Pathways Found in Prostate Cancer 1. 2. 1 1 1 1 Gordon M. Cann , Zulfiqar G. Gulzar , Samantha Cooper , Robin Li , Shujun Luo , Mai Tat , 1 1 3 1 2 Sarah Stuart , Gary Schroth , Sandhya Srinivas , Mostafa Ronaghi *, James D. Brooks *, AmirAli H. Talasaz1* 1 Department of Diagnostic Research, Illumina, Inc., Hayward, California, United States of America, 2 Department of Urology, Stanford University Medical Center, Stanford, California, United States of America, 3 Department of Medicine, Division of Oncology, Stanford University Medical Center, Stanford, California, United States of America Abstract Circulating tumor cells (CTC) mediate metastatic spread of many solid tumors and enumeration of CTCs is currently used as a prognostic indicator of survival in metastatic prostate cancer patients. Some evidence suggests that it is possible to derive additional information about tumors from expression analysis of CTCs, but the technical difficulty of isolating and analyzing individual CTCs has limited progress in this area. To assess the ability of a new generation of MagSweeper to isolate intact CTCs for downstream analysis, we performed mRNA-Seq on single CTCs isolated from the blood of patients with metastatic prostate cancer and on single prostate cancer cell line LNCaP cells spiked into the blood of healthy donors. We found that the MagSweeper effectively isolated CTCs with a capture efficiency that matched the CellSearch platform. However, unlike CellSearch, the MagSweeper facilitates isolation of individual l
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