molecular evolution of the deuterolysin (m35) family genes in coccidioides分子进化的deuterolysin(国防军m35)投入正式家族基因在球孢子菌属.pdfVIP

Molecular Evolution of the Deuterolysin (M35) Family Genes in Coccidioides Juan Li., Li Yu., Yanmei Tian, Ke-Qin Zhang* Laboratory for Conservation and Utilization of Bio-resources, and Key Laboratory for Microbial Resources of the Ministry of Education, Yunnan University, Kunming, People’s Republic of China Abstract Coccidioides is a primary fungal pathogen of humans, causing life-threatening respiratory disease known as coccidioidomycosis (Valley fever) in immunocompromised individuals. Recently, Sharpton et al (2009) found that the deuterolysin (M35) family genes were significantly expanded in both the Coccidioides genus and in U. reesii, and that Coccidioides has acquired three more M35 family genes than U. reesii. In the present work, phylogenetic analyses based on a total of 28 M35 family genes using different alignments and tree-building methods consistently revealed five clades with high nodal supports. Interestingly, likelihood ratio tests suggested significant differences in selective pressure on the ancestral lineage of three additional duplicated M35 family genes from Coccidioides species compared to the other lineages in the phylogeny, which may be associated with novel functional adaptations of M35 family genes in the Coccidioides species, e.g., recent pathogenesis acquisition. Our study adds to the expanding view of M35 family gene evolution and functions as well as establishes a theoretical foundation for future experimental investigations. Citation: Li J, Yu L, Tian Y, Zhang K-Q (2012) Molecular Evolution of the Deuterolysin (M35) Family Genes in Coccidioides. PLoS ONE 7(2): e31536. doi:10.1371/ journal.pone.0031536 Editor: David Liberles, University of Wyoming, United States of America Received September 13, 2011; Accepte