humanized mouse model of ovarian cancer recapitulates patient solid tumor progression, ascites formation, and metastasis人源化小鼠模型的卵巢癌病人固体肿瘤恶化,立在腹水的形成和转移.pdfVIP

Humanized Mouse Model of Ovarian Cancer Recapitulates Patient Solid Tumor Progression, Ascites Formation, and Metastasis 1 2 3 4 1 Richard B. Bankert *, Sathy V. Balu-Iyer , Kunle Odunsi , Leonard D. Shultz , Raymond J. Kelleher Jr. , 1 1¤ 1 1 Jennifer L. Barnas , Michelle Simpson-Abelson , Robert Parsons , Sandra J. Yokota 1 Department of Microbiology and Immunology, The State University of New York, University at Buffalo, Buffalo, New York, United States of America, 2 Pharmaceutical Sciences, The State University of New York, University at Buffalo, Amherst, New York, United States of America, 3 Department of Gynecologic Oncology, Roswell Park Cancer Institute, Buffalo, New York, United States of America, 4 Jackson Laboratory, Bar Harbor, Maine, United States of America Abstract Ovarian cancer is the most common cause of death from gynecological cancer. Understanding the biology of this disease, particularly how tumor-associated lymphocytes and fibroblasts contribute to the progression and metastasis of the tumor, has been impeded by the lack of a suitable tumor xenograft model. We report a simple and reproducible system in which the tumor and tumor stroma are successfully engrafted into NOD-scid IL2Rcnull (NSG) mice. This is achieved by injecting tumor cell aggregates derived from fresh ovarian tumor biopsy tissues (including tumor cells, and tumor-associated lymphocytes and fibroblasts) i.p. into NSG mice. Tumor progression in these mice closely parallels many of the events that are observed in ovarian cancer patients. Tumors establish in the omentum, ova