hippocampal deletion of bdnf gene attenuates gamma oscillations in area ca1 by up-regulating 5-ht3 receptor海马bdnf基因的删除变弱伽马振荡在ca1区调控5-ht3受体.pdfVIP

Hippocampal Deletion of BDNF Gene Attenuates Gamma Oscillations in Area CA1 by Up-Regulating 5-HT3 Receptor 1,2 1 Ying Huang , Alexei Morozov * 1 Unit on Behavioral Genetics, Laboratory of Molecular Pathophysiology, National Institute of Mental Health, National Institutes of Health, Bethesda, Maryland, United States of America, 2 Department of Physiology and Pathophysiology, Shanghai Medical College, Fudan University, Shanghai, China Abstract Background: Pyramidal neurons in the hippocampal area CA3 express high levels of BDNF, but how this BDNF contributes to oscillatory properties of hippocampus is unknown. Methodology/Principal Findings: Here we examined carbachol-induced gamma oscillations in hippocampal slices lacking BDNF gene in the area CA3. The power of oscillations was reduced in the hippocampal area CA1, which coincided with increases in the expression and activity of 5-HT3 receptor. Pharmacological block of this receptor partially restored power of gamma oscillations in slices from KO mice, but had no effect in slices from WT mice. Conclusion/Significance: These data suggest that BDNF facilitates gamma oscillations in the hippocampus by attenuating signaling through 5-HT3 receptor. Thus, BDNF modulates hippocampal oscillations through serotonergic system. Citation: Huang Y, Morozov A (2011) Hippocampal Deletion of BDNF Gene Attenuates Gamma Oscillations in Area CA1 by Up-Regulating 5-HT3 Receptor. PLoS ONE 6(1): e16480. doi:10.1371/journal.pone.0016480 Editor: Kenji Hashimoto, Chiba University Center for Forensic Mental Health, Japan Received August 27, 2010; Accepted December 21, 2010; Published January 26, 2011 This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration which stipulates that, once placed in the public domain, this work may be freely reproduced, di