high-resolution analysis of the 5′-end transcriptome using a next generation dna sequencer高分辨率的5u2032端转录组分析使用下一代dna测序仪.pdfVIP

High-Resolution Analysis of the 59-End Transcriptome Using a Next Generation DNA Sequencer 1. 2. 2 1 2 2 Shin-ichi Hashimoto , Wei Qu , Budrul Ahsan , Katsumi Ogoshi , Atsushi Sasaki , Yoichiro Nakatani , 1 1 1 3 3 4 Yongjun Lee , Masako Ogawa , Akio Ametani , Yutaka Suzuki , Sumio Sugano , Clarence C. Lee , 4 2 1 Robert C. Nutter , Shinichi Morishita , Kouji Matsushima * 1 Department of Molecular Preventive Medicine, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan, 2 Department of Computational Biology, Graduate School of Frontier Sciences, The University of Tokyo, Kashiwa, Japan, 3 Department of Medical Genome Science, Graduate School of Frontier Science, The University of Tokyo, Kashiwa, Japan, 4 Applied Biosystems, Foster City, California, United States of America Abstract Massively parallel, tag-based sequencing systems, such as the SOLiD system, hold the promise of revolutionizing the study of whole genome gene expression due to the number of data points that can be generated in a simple and cost-effective manner. We describe the development of a 59–end transcriptome workflow for the SOLiD system and demonstrate the advantages in sensitivity and dynamic range offered by this tag-based application over traditional approaches for the study of whole genome gene expression. 59-end transcriptome analysis was used to study whole genome gene expression within a colon cancer cell line, HT-29, treated with the DNA methyltransferase inhibitor, 5-aza-29-deoxycytidine (5Aza). More than