high viral load of human bocavirus correlates with duration of wheezing in children with severe lower respiratory tract infection高病毒载量的人类bocavirus与儿童喘息的时间严重的下呼吸道感染.pdfVIP

High Viral Load of Human Bocavirus Correlates with Duration of Wheezing in Children with Severe Lower Respiratory Tract Infection 1 4 2 1 1 3 1 Yu Deng , Xiaoyang Gu , Xiaodong Zhao , Jian Luo , Zhengxiu Luo , Lijia Wang , Zhou Fu , 2 1 Xiqiang Yang , Enmei Liu * 1 Department of Respiratory Medicine, Children’s Hospital, Chongqing Medical University, Chongqing, People’s Republic of China, 2 Department of Nephrology and Immunology Medicine, Children’s Hospital, Chongqing Medical University, Chongqing, People’s Republic of China, 3 Laboratory of Children’s Respiratory Medicine, Ministry of Education Key Laboratory of Child Development and Disorders, Chongqing Medical University, Chongqing, People’s Republic of China, 4 Paediatric unit of the Bethune International Peace Hospital of the PLA, Shijiazhuang Hebei Province, People’s Republic of China Abstract Background: Human bocavirus (HBoV) is a newly discovered parvovirus and increasing evidences are available to support its role as an etiologic agent in lower respiratory tract infection (LRTI). The objective of this study is to assess the impact of HBoV viral load on clinical characteristics in children who were HBoV positive and suffered severe LRTI. Methods: Lower respiratory tract aspirates from 186 hospitalized children with severe LRTI were obtained by bronchoscopy. HBoVs were detected by real-time PCR and other 10 infectious agents were examined using PCR and/or direct fluorescent assay. Results: Thirty-one patients (24.6%) were tested positive for HBoV in the respiratory tract aspirates. Fifteen samples had a high viral load (.104 4