gsk3 inhibitor-bio regulates proliferation of immortalized pancreatic mesenchymal stem cells (ipmscs)gsk3 inhibitor-bio调节扩散不灭的胰腺间充质干细胞(ipmscs).pdfVIP

GSK3 Inhibitor-BIO Regulates Proliferation of Immortalized Pancreatic Mesenchymal Stem Cells (iPMSCs) 1,2 1 1 1 1 3 3 1 Hui Cao , Yuankui Chu , Xiao Lv , Pubin Qiu , Chao Liu , Huiru Zhang , Dan Li , Sha Peng , Zhongying 1 1 Dou , Jinlian Hua * 1 College of Veterinary Medicine, Shaanxi Center of Stem Cells Engineering and Technology, Key Lab for Animal Biotechnology of Agriculture Ministry of China, Northwest A F University, Yangling, Shaanxi, People’s Republic of China, 2 Hope Bio-technology (Su Zhou) Co., Ltd., Su Zhou, Jiangsu, People’s Republic of China, 3 College of Bioengineering, Henan University of Technology, Zhengzhou Henan, People’s Republic of China Abstract Background: The small molecule 6-bromoindirubin-30-oxime (BIO), a glycogen synthase kinase 3 (GSK3) inhibitor, is a pharmacological agent known to maintain self-renewal in human and mouse embryonic stem cells (ESCs). However, the precise role of GSK3 in immortalized pancreatic mesenchymal stem cells (iPMSCs) growth and survival is not completely understood at present. Results: To determine whether this molecule is involved in controlling the proliferation of iPMSCs, we examined the effect of BIO on iPMSCs. We found that the inactivation of GSK3 by BIO can robustly stimulate iPMSCs proliferation and mass formation as shown by QRT-PCR, western blotting, 5-Bromo-2-deoxyuridine (BrdU) immunostaining assay and tunel assay. However, we did not find the related roles of BIO on b cell differentiation by immunostaining, QRT-PCR assay, glucose- stimulated insulin release and C-peptide content analysis. Conclusions: These results suggest that BIO plays a key role in the regulation of cell mass proliferation and maintenance of