gi-coupled gpcr signaling controls the formation and organization of human pluripotent coloniesgi-coupled gpcr信号控制人类多能殖民地的形成和组织.pdfVIP

G -Coupled GPCR Signaling Controls the Formation and i Organization of Human Pluripotent Colonies 1,2 1 1 1,3 1,2,4 Kenta Nakamura *, Nathan Salomonis , Kiichiro Tomoda , Shinya Yamanaka , Bruce R. Conklin 1 Gladstone Institute of Cardiovascular Disease, San Francisco, California, United States of America, 2 Department of Medicine, University of California San Francisco, San Francisco, California, United States of America, 3 Center for iPS Cell Research and Application, Kyoto University, Kyoto, Japan, 4 Department of Cellular and Molecular Pharmacology, University of California San Francisco, San Francisco, California, United States of America Abstract Background: Reprogramming adult human somatic cells to create human induced pluripotent stem (hiPS) cell colonies involves a dramatic morphological and organizational transition. These colonies are morphologically indistinguishable from those of pluripotent human embryonic stem (hES) cells. G protein-coupled receptors (GPCRs) are required in diverse developmental processes, but their role in pluripotent colony morphology and organization is unknown. We tested the hypothesis that Gi-coupled GPCR signaling contributes to the characteristic morphology and organization of human pluripotent colonies. Methodology/Principal Findings: Specific and irreversible inhibition of G -coupled GPCR signaling by pertussis toxin i markedly altered pluripotent colony morphology. Wild-