gibberellin and auxin influence the diurnal transcription pattern of photoreceptor genes via cry1a in tomato赤霉素和生长素影响昼夜感光基因的转录模式通过cry1a番茄.pdfVIP

Gibberellin and Auxin Influence the Diurnal Transcription Pattern of Photoreceptor Genes via CRY1a in Tomato 1 1 2 1 Paolo Facella , Loretta Daddiego , Giovanni Giuliano , Gaetano Perrotta * 1 Italian National Agency for New Technologues, Energy and Sustainable Economic Development (ENA), Trisaia Research Center, Rotondella, Italy, 2 Italian National Agency for New Technologues, Energy and Sustainable Economic Development (ENA), Casaccia Research Center, Rome, Italy Abstract Background: Plant photoreceptors, phytochromes and cryptochromes, regulate many aspects of development and growth, such as seed germination, stem elongation, seedling de-etiolation, cotyledon opening, flower induction and circadian rhythms. There are several pieces of evidence of interaction between photoreceptors and phyto-hormones in all of these physiological processes, but little is known about molecular and genetic mechanisms underlying hormone-photoreceptor crosstalk. Methodology/Principal Findings: In this work, we investigated the molecular effects of exogenous phyto-hormones to photoreceptor gene transcripts of tomato wt, as well as transgenic and mutant lines with altered cryptochromes, by monitoring day/night transcript oscillations. GA and auxin alter the diurnal expression level of different photoreceptor genes in tomato, especially in mutants that lack a working form of cryptochrome 1a: in those mutants the expression of some (IAA) or most (GA) photoreceptor genes is down regulated by these hormones. Conclusions/Significance: Our results highlight the presence of molecular relationships among cryptochrome 1a protein, hormones, and photoreceptors’ gene expression in tomato, suggesting th