germ cell transplantation using sexually competent fish an approach for rapid propagation of endangered and valuable germlines用性生殖细胞移植主管鱼的方法中快速传播的濒危和有价值.pdfVIP

Germ Cell Transplantation Using Sexually Competent Fish: An Approach for Rapid Propagation of Endangered and Valuable Germlines ¨ Sullip K. Majhi, Ricardo S. Hattori, Masashi Yokota, Seiichi Watanabe, Carlos A. Strussmann* Department of Marine Bioscience, Tokyo University of Marine Science and Technology, Tokyo, Japan Abstract The transplantation of germ cells into adult recipient gonads is a tool with wide applications in animal breeding and conservation of valuable and/or endangered species; it also provides a means for basic studies involving germ cell (GC) proliferation and differentiation. Here we describe the establishment of a working model for xenogeneic germ cell transplantation (GCT) in sexually competent fish. Spermatogonial cells isolated from juveniles of one species, the pejerrey Odontesthes bonariensis (Atherinopsidae), were surgically transplanted into the gonads of sexually mature Patagonian pejerrey O. hatcheri, which have been partially depleted of endogenous GCs by a combination of Busulfan (40 mg/kg) and high water temperature (25uC) treatments. The observation of the donor cells’ behavior showed that transplanted spermatogonial cells were able to recolonize the recipients’ gonads and resume spermatogenesis within 6 months from the GCT. The presence of donor-derived gametes was confirmed by PCR in 20% of the surrogate O. hatcheri fathers at 6 months and crosses with O. bonariensis mothers produced hybrids and pure O. bonariensis, with donor-derived germline transmission rates of 1.2–13.3%. These findings indicate that transplantation of spermatogonial cel