gep100-arf6-amap1-cortactin pathway frequently used in cancer invasion is activated by vegfr2 to promote angiogenesisgep100-arf6-amap1-cortactin通路经常用于癌症入侵是由vegfr2激活促进血管生成.pdfVIP

GEP100-Arf6-AMAP1-Cortactin Pathway Frequently Used in Cancer Invasion Is Activated by VEGFR2 to Promote Angiogenesis 1,2 1,2 3 3 2,4,5 5,6 Ari Hashimoto , Shigeru Hashimoto , Ryo Ando , Kosuke Noda , Eiji Ogawa , Hirokazu Kotani , 7 2,5 2,8 4 6 Mayumi Hirose , Toshi Menju , Masaki Morishige , Toshiaki Manabe , Yoshinobu Toda , Susumu Ishida3, Hisataka Sabe1,2* 1 Department of Molecular Biology, Hokkaido University Graduate School of Medicine, Sapporo, Japan, 2 Department of Molecular Biology, Osaka Bioscience Institute, Suita, Osaka, Japan, 3 Department of Ophthalmology, Hokkaido University Graduate School of Medicine, Sapporo, Japan, 4 Laboratory of Diagnostic Pathology, Faculty of Medicine, Kyoto University, Kyoto, Japan, 5 Department of Thoracic Surgery, Faculty of Medicine, Kyoto University, Kyoto, Japan, 6 Center for Anatomical Studies, Kyoto University Graduate School of Medicine, Kyoto, Japan, 7 Laboratory of Supramolecular Crystallography, Institute for Protein Research, Osaka University, Osaka, Japan, 8 Department of Neurosurgery, School of Medicine, Oita University, Oita, Japan Abstract Angiogenesis and cancer invasiveness greatly contribute to cancer malignancy. Arf6 and its effector, AMAP1, are frequently overexpressed in breast cancer, and constitute a central pathway to induce the invasion and metastasis. In this pathway, Arf6 is activated by EGFR via GEP100. Arf6 is highly expressed also in human umbilical vein endothelial cells (HUVECs) and is implicated in angiogenesis. Her