genome-wide association study identifies candidate genes that affect plant height in chinese elite maize (zea mays l.) inbred lines全基因组关联研究确定候选基因影响株高在中国精英玉米(玉米l .).pdfVIP
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genome-wide association study identifies candidate genes that affect plant height in chinese elite maize (zea mays l.) inbred lines全基因组关联研究确定候选基因影响株高在中国精英玉米(玉米l .)
Genome-Wide Association Study Identifies Candidate Genes That Affect Plant Height in Chinese Elite Maize (Zea mays L.) Inbred Lines Jianfeng Weng., Chuanxiao Xie., Zhuanfang Hao., Jianjun Wang, Changlin Liu, Mingshun Li, Degui Zhang, Li Bai, Shihuang Zhang*, Xinhai Li* Institute of Crop Science, Chinese Academy of Agricultural Sciences, The National Key Facility for Crop Gene Resources and Genetic Improvement, Beijing, China Abstract Background: The harvest index for many crops can be improved through introduction of dwarf stature to increase lodging resistance, combined with early maturity. The inbred line Shen5003 has been widely used in maize breeding in China as a key donor line for the dwarf trait. Also, one major quantitative trait locus (QTL) controlling plant height has been identified in bin 5.05–5.06, across several maize bi-parental populations. With the progress of publicly available maize genome sequence, the objective of this work was to identify the candidate genes that affect plant height among Chinese maize inbred lines with genome wide association studies (GWAS). Methods and Findings: A total of 284 maize inbred lines were genotyped using over 55,000 evenly spaced SNPs, from which a set of 41,101 SNPs were filtered with stringent quality control for further data analysis. With the population structure controlled in a mixed linear model (MLM) implemented with the software TASSEL, we carried out a genome-wide association study (GWAS) for plant height. A total of 204 SNPs (P#0.0001) and 105 genomic loci harboring coding regions were identified. Four loci containing genes associated with gibberellin (GA), auxin, and epigenetic pathways may be involved in natural variation that led to a dwarf phenotype in elite maize inbred lines. Among them, a favorable allele for dwarfing on chromosome 5 (SNP PZE-105
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