有哪些信誉好的足球投注网站generating a knockdown transgene against drosophila heterochromatic tim17b gene encoding mitochondrial translocase subunit生成一个可拆卸的转基因对果蝇异色的tim17b基因编码线粒体移位酶亚基.pdfVIP
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generating a knockdown transgene against drosophila heterochromatic tim17b gene encoding mitochondrial translocase subunit生成一个可拆卸的转基因对果蝇异色的tim17b基因编码线粒体移位酶亚基
Generating a Knockdown Transgene against Drosophila Heterochromatic Tim17b Gene Encoding Mitochondrial Translocase Subunit . . Mikael Garabedian , Michael Jarnik , Elena Kotova, Alexei V. Tulin* Fox Chase Cancer Center, Philadelphia, Pennsylvania, United States of America Abstract Heterochromatic regions of eukaryotic genomes contain multiple functional elements involved in chromosomal dynamics, as well as multiple housekeeping genes. Cytological and molecular peculiarities of heterochromatic loci complicate genetic studies based on standard approaches developed using euchromatic genes. Here, we report the development of an RNAi- based knockdown transgenic construct and red fluorescent reporter transgene for a small gene, Tim17b, which localizes in constitutive heterochromatin of Drosophila melanogaster third chromosome and encodes a mitochondrial translocase subunit. We demonstrate that Tim17b protein is required strictly for protein delivery to mitochondrial matrix. Knockdown of Tim17b completely disrupts functions of the mitochondrial translocase complex. Using fluorescent recovery after photobleaching assay, we show that Tim17b protein has a very stable localization in the membranes of the mitochondrial network and that its exchange rate is close to zero when compared with soluble proteins of mitochondrial matrix. These results confirm that we have developed comprehensive tools to study functions of heterochromatic Tim17b gene. Citation: Garabedian M, Jarnik M, Kotova E, Tulin AV (2011) Generating a Knockdown Transgene against Drosophila Heterochromatic Tim17b Gene Encoding Mitochondrial Translocase Subunit. PLoS ONE 6(10): e25945. doi:10.1371/journal.pone.0025945 Editor: Charalampos Babis Spilianakis, University of Crete, Greece Received May 31, 2011; A
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