genetic and physical mapping of candidate genes for resistance to fusarium oxysporum f.sp. tracheiphilum race 3 in cowpea [vigna unguiculata (l.) walp]候选基因的遗传和物理映射为尖孢镰刀菌耐药性f.sp。.pdfVIP
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genetic and physical mapping of candidate genes for resistance to fusarium oxysporum f.sp. tracheiphilum race 3 in cowpea [vigna unguiculata (l.) walp]候选基因的遗传和物理映射为尖孢镰刀菌耐药性f.sp。
Genetic and Physical Mapping of Candidate Genes for Resistance to Fusarium oxysporum f.sp. tracheiphilum Race 3 in Cowpea [Vigna unguiculata (L.) Walp] 1 1 1 1,2 3 Marti Pottorff , Steve Wanamaker , Yaqin Q. Ma , Jeffrey D. Ehlers , Philip A. Roberts , Timothy J. Close1* 1 Department of Botany Plant Sciences, University of California Riverside, Riverside California, United States of America, 2 Bill Melinda Gates Foundation, Seattle, Washington, United States of America, 3 Department of Nematology, University of California Riverside, Riverside, California, United States of America Abstract Fusarium oxysporum f.sp. tracheiphilum (Fot) is a soil-borne fungal pathogen that causes vascular wilt disease in cowpea. Fot race 3 is one of the major pathogens affecting cowpea production in California. Identification of Fot race 3 resistance determinants will expedite delivery of improved cultivars by replacing time-consuming phenotypic screening with selection based on perfect markers, thereby generating successful cultivars in a shorter time period. Resistance to Fot race 3 was studied in the RIL population California Blackeye 27 (resistant) x 24-125B-1 (susceptible). Biparental mapping identified a Fot race 3 resistance locus, Fot3-1, which spanned 3.56 cM on linkage group one of the CB27 x 24-125B-1 genetic map. A marker-trait association narrowed the resistance locus to a 1.2 cM region and identified SNP marker 1_1107 as co- segregating with Fot3-1 resistance. Macro and microsynteny was observed for the Fot3-1 locus region in Glycine max where six disease resistance genes were observed in the two syntenic regions of soybean chromosome
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