gene pyramiding of peptidase inhibitors enhances plant resistance to the spider mite tetranychus urticae基因成金字塔形状的肽酶抑制剂提高植物抵抗红蜘蛛测定.pdfVIP

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gene pyramiding of peptidase inhibitors enhances plant resistance to the spider mite tetranychus urticae基因成金字塔形状的肽酶抑制剂提高植物抵抗红蜘蛛测定.pdf

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gene pyramiding of peptidase inhibitors enhances plant resistance to the spider mite tetranychus urticae基因成金字塔形状的肽酶抑制剂提高植物抵抗红蜘蛛测定

Gene Pyramiding of Peptidase Inhibitors Enhances Plant Resistance to the Spider Mite Tetranychus urticae 1,2,3 ´ 1 1 ´ Maria Estrella Santamaria , Ines Cambra, Manuel Martinez , Clara Pozancos , Pablo Gonzalez- 1 2 ˜ 3 3 1 Melendi , Vojislava Grbic , Pedro Castanera , Felix Ortego , Isabel Diaz * ´ ´ ´ 1 Centro de Biotecnologıa y Genomica de Plantas (UPM-INIA). Campus Montegancedo Universidad Politecnica de Madrid, Autopista M40 (km 38), Madrid, Spain, ´ 2 Department of Biology Western University, Ontario, Canada, 3 Dpto. Biologia Medioambiental, Centro de Investigaciones Biologicas, CSIC, Madrid, Spain Abstract The two-spotted spider mite Tetranychus urticae is a damaging pest worldwide with a wide range of host plants and an extreme record of pesticide resistance. Recently, the complete T. urticae genome has been published and showed a proliferation of gene families associated with digestion and detoxification of plant secondary compounds which supports its polyphagous behaviour. To overcome spider mite adaptability a gene pyramiding approach has been developed by co- expressing two barley proteases inhibitors, the cystatin Icy6 and the trypsin inhibitor Itr1 genes in Arabidopsis plants by Agrobacterium-mediated transformation. The presence and expression of both transgenes was studied by conventional and quantitative real time RT-PCR