a water soluble coq10 formulation improves intracellular distribution and promotes mitochondrial respiration in cultured cells水溶性辅酶q配方提高细胞内的分布和促进培养细胞中线粒体呼吸.pdfVIP

A Water Soluble CoQ10 Formulation Improves Intracellular Distribution and Promotes Mitochondrial Respiration in Cultured Cells 1 1 2 1 1 Christian Bergamini , Noah Moruzzi , Antonella Sblendido , Giorgio Lenaz , Romana Fato * 1 Department of Biochemistry ‘‘G. Moruzzi’’, University of Bologna, Bologna, Italy, 2 Scharper Therapeutics, Medical Department, Sesto S. Giovanni, Milano, Italy Abstract Background: Mitochondria are both the cellular powerhouse and the major source of reactive oxygen species. Coenzyme Q10 plays a key role in mitochondrial energy production and is recognized as a powerful antioxidant. For these reasons it can be argued that higher mitochondrial ubiquinone levels may enhance the energy state and protect from oxidative stress. Despite the large number of clinical studies on the effect of CoQ10 supplementation, there are very few experimen- tal data about the mitochondrial ubiquinone content and the cellular bioenergetic state after supplementation. Controversial clinical and in vitro results are mainly due to the high hydrophobicity of this compound, which reduces its bioavailability. Principal Findings: We measured the cellular and mitochondrial ubiquinone content in two cell lines (T67 and H9c2) after supplementation with a hydrophilic CoQ10 formulation (QterH) and native CoQ10. Our results show that the water soluble formulation is more efficient in increasing ubiquinone levels. We have evaluated the bioenergetics effect of ubiquinone treatment, demonstrating that intracellular CoQ10 content after Qter supplementation positively correlates with an improved mitochondrial functionality (increased oxygen consumption rate, transmembra