a secure semi-field system for the study of aedes aegypti一个安全的半实地系统研究埃及伊蚊.pdfVIP

A Secure Semi-Field System for the Study of Aedes aegypti 1 1 1 2 3 Scott A. Ritchie *, Petrina H. Johnson , Anthony J. Freeman , Robin G. Odell , Neal Graham , Paul A. 4 5 6 7 DeJong , Graeme W. Standfield , Richard W. Sale , Scott L. O’Neill 1 School of Public Health, Tropical Medicine and Rehabilitation Sciences, James Cook University, Cairns, Queensland, Australia, 2 About Awnings, Gordonvale, Queensland, Australia, 3 Power, Graham and Dempsey Pty Ltd Architects, Cairns, Queensland, Australia, 4 Hutchisons Builders Pty Ltd, Smithfield, Queensland, Australia, 5 MGF Consultants (NQ) Pty Ltd, Cairns, Queensland, Australia, 6 S2F Pty Ltd, Brisbane, Queensland, Australia, 7 School of Biological Sciences, The University of Queensland, St Lucia, Queensland, Australia Abstract Background: New contained semi-field cages are being developed and used to test novel vector control strategies of dengue and malaria vectors. We herein describe a new Quarantine Insectary Level-2 (QIC-2) laboratory and field cages (James Cook University Mosquito Research Facility Semi-Field System; MRF SFS) that are being used to measure the impact of the endosymbiont Wolbachia pipientis on populations of Aedes aegypti in Cairns Australia. Methodology/Principal Findings: The MRF consists of a single QIC-2 laboratory/insectary that connects through a central corridor to two identical QIC-2 semi-field cages. The semi-field cages are constructed of two layers of 0.25 mm stainless steel wire mesh to prevent escape of mosquitoes and ingress of other insects. The cages are covered by an aluminum security mesh to prevent penetration of the cages b