a high-throughput forward genetic screen identifies genes required for virulence of pseudomonas syringae pv. maculicola es4326 on arabidopsis向前一个高通量基因所需的屏幕识别基因丁香假单胞菌的毒性。.pdfVIP

A High-Throughput Forward Genetic Screen Identifies Genes Required for Virulence of Pseudomonas syringae pv. maculicola ES4326 on Arabidopsis 1¤ 1 1 1 1 1,2 Karl J. Schreiber , David Ye , Eric Fich , Allen Jian , Timothy Lo , Darrell Desveaux * 1 Department of Cell and Systems Biology, University of Toronto, Toronto, Ontario, Canada, 2 Centre for the Analysis of Genome Evolution and Function, University of Toronto, Toronto, Ontario, Canada Abstract Successful pathogenesis requires a number of coordinated processes whose genetic bases remain to be fully characterized. We utilized a high-throughput, liquid media-based assay to screen transposon disruptants of the phytopathogen Pseudomonas syringae pv. maculicola ES4326 to identify genes required for virulence on Arabidopsis. Many genes identified through this screen were involved in processes such as type III secretion, periplasmic glucan biosynthesis, flagellar motility, and amino acid biosynthesis. A small set of genes did not fall into any of these functional groups, and their disruption resulted in context-specific effects on in planta bacterial growth. Citation: Schreiber KJ, Ye D, Fich E, Jian A, Lo T, et al. (2012) A High-Throughput Forward Genetic Screen Identifies Genes Required for Virulence of Pseudomonas syringae pv. maculicola ES4326 on Arabidopsis. PLoS ONE 7(8): e41461. doi:10.1371/journal.pone.0041461 Editor: Szabolcs Semsey, Niels Bohr Institute, Denmark Received May 15, 2012; Accepted June 21, 2012; Published August 1, 2012 Copyright: 2012 Schreiber et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits