biacore 3000生物分子相互作用分析仪原理和操作注意事项（Principle and operation notes of Biacore 3000 biomolecular interaction analyzer）.docVIP

biacore 3000生物分子相互作用分析仪原理和操作注意事项（Principle and operation notes of Biacore 3000 biomolecular interaction analyzer） BIAcore 3000 bio molecular interaction analyzer principle and operation precautions BIA (Biomolecular Interaction Analysis) provides the interaction between biological molecules in real time observation technology. It can through the specific observation of two molecules, with two molecules can know how strong, but also to understand the combination of biological molecules of the total number of collaborative and participants. BIA can be obtained by other methods to get the results, because it can reflect the real-time binding of molecules in the process of changing every second. Without the determination of BIA is widely used in various types of biological systems with the analysis of markers, from all kinds of small molecules, peptides, proteins, oligonucleotides and chitosan until lipid, phage, virus and cell. BIA is the use of metal film surface refraction rate change, change of resonance angle, to infer the changes of metal film surface. The first is a kind of biological molecules immobilized on the sensor chip surface, and the molecular interactions in the solution flows through the surface of the chip. Can change detector combination, tracking and detection in solution and molecular chip surface molecular dissociation of the whole process. BIA technology is a kind of surface plasmon resonance (SPR) technology based on the analysis of the physical phenomena of optical biosensors. Without the use of fluorescence labeling and isotope labeling, thus maintaining the natural activity of biological molecules. When the incident light to a critical angle to two kinds of transparent medium interface will have a total reflection, and the reflected light intensity at each point should be the same, but if the plating on the dielectric surface a layer of metal film, because the incident light can cause resonance of free electrons in the metal, which lead to the