液相色谱仪常用检测器的种类（Types of commonly used detectors in liquid chromatography）.docVIP

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液相色谱仪常用检测器的种类（Types of commonly used detectors in liquid chromatography）.doc

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液相色谱仪常用检测器的种类（Types of commonly used detectors in liquid chromatography）

液相色谱仪常用检测器的种类（Types of commonly used detectors in liquid chromatography） The function of the detector is to change the composition and content of the sample in the column effluent into the signals to be detected. The common detectors include ultraviolet absorption, fluorescence, differential refraction, chemiluminescence, etc.. 1. UV Vis detector (Ultravioletvisible detector, UVD) Ultraviolet visible absorption detector (UVD) is one of the most widely used detectors in HPLC. Almost all liquid chromatography is equipped with this kind of detector. It is characterized by high sensitivity, wide linear range, low noise, suitable for gradient elution of strong absorption material detection limit is 1ng, after the detection without damage of the sample, can be used in the preparation, and can be used in tandem with any detector. The principle and structure of an ultraviolet visible detector is similar to that of a general spectrophotometer. In fact, it is an ultraviolet visible photometer with a flow field. (1) UV absorption detector Light ultraviolet absorption detector commonly used deuterium lamp, deuterium lamp can emit a continuous wavelength ultraviolet visible range, and the installation of a grating monochromator, the wide spectrum (190nm ~ 800nm). It has two circulation pool, a reference, a measurement, the light emitted from the ultraviolet radiation to the circulation pool, if the two circulation pool by homogeneous pure solvent, they in the UV wavelength almost no absorption, the radiation intensity is equal to the phototube received, no output signal. When a component enters a measuring cell, a certain amount of ultraviolet light is absorbed so that the intensity of the radiation received by the two photoelectric tube is not equal, and then the signal output is obtained, and the output signal size is related to the concentration of the component. Limitations: the selection of mobile phase is restricted, the solvent cannot has ultraviolet absorption of mobile