免疫性卵巢早衰小鼠生殖能力探究.docVIP

免疫性卵巢早衰小鼠生殖能力探究[摘要] 目的：探讨补肾复方左归丸对免疫性卵巢早衰（POF）小鼠动情周期、卵泡刺激素（FSH）、雌二醇（E2）、受孕率的影响。方法：以小鼠透明带3为抗原，皮下多点注射免疫BALB/c雌性小鼠建立免疫性卵巢早衰模型。设左归丸低、中、高不同剂量进行治疗，以泼尼松、己烯雌酚为阳性对照，观察动情周期、受孕率，ELISA法检测外周血清FSH、E2水平。结果：模型组小鼠动情周期紊乱，FSH增高，E2降低，受孕率低下。左归丸各剂量组动情周期时间延长，但仍有规律性变化。FSH升高及E2降低均不明显，受孕率也低下。结论：模型组卵巢功能损伤、破坏。左归丸能改善、保护卵巢功能，但对受孕率改善不明显。 [关键词] 卵巢早衰；左归丸；动情周期；FSH；E2；受孕率 [中图分类号] R711[文献标识码]A [文章编号]1673-7210（2008）02（c）-013-03 Reproductive capacity of immune premature ovarian failure mice ZHU Ling, LUO Song-ping, XU Li-mian （Department of Gynecology，The First Affiliated Hospital，Guangzhou University of TCM ，Guangzhou510405, China） [Abstract] Objective: To probe the effect of Zuogui Pill (ZGP), a Chinese compound recipe for tonifying Shen, on estrous cycle, FSH、E2 and conception rate of mice. in mice with immune premature ovarian failure (POF).Methods:BALB/c female mice model of POF was established by multiple sites subcutaneous injection of zona pellucida 3 of mice, and treated with different dosage ZGP of low, middle and high one, with prednisone and diaethylstilbestrol as positive control. Observed estrous cycle of mice and conception rate. Detected FSH and E2 in peripheral blood serum by ELISA. Results:The estrous cycle of POF models were disorder. The level of FSH increased and E2 decreased. The conception rate become low. The period of estrous cycle prolonged in the mice of different dosage ZGP, but still kept regularity period. The change of FSH and E2 is not significant. The conception rate was lower also. Conclusion:The ovarian function is hurt and destroyed in immune POF mice. ZGP can improve and preserve the ovarian function, but not improve significantly conception rate. [Key words] Premature ovarian failure; Zuogui Pill; Estrous cycle; FSH; E2; Conception rate 卵巢早衰（premature ovarian failure, POF）是指女性在40岁前出现卵巢功能衰退，主要表现为月经稀发或持续闭经，常伴烘热、多汗、失眠等躯体症状，及出现焦虑、抑郁、敏感、敌对、压抑，阴道干涩，性交痛、性生活不满意等不适[1]。骨质疏松、心血管疾病等患者发生可较自然绝经者提前10年，妊娠主要依赖卵子捐赠[2]，是一类严重影响妇女身心健康的疾病。左归丸具有补肾精、养精血、化瘀血之功，临床治疗卵巢早