大蒜素对铜绿假单胞菌生物膜早期黏附及胞外多糖的影响.pdfVIP

2009 1 21 1 9 : 1005-376 ( 2009) 01-0009-04 = 林丽华,余加林, 林雅茵, 刘官信 (重庆医科大学附属儿童医院新生儿科, 重庆 400014) = PA01( biofilm, BF) (Ex- tracellular Polymeric Substances, EPS) 96 pGF- Puv PA01, , ; A ( FITC conjugated concanavalin A, FITC-conA) EPS, EPS; - EPS 6 h, 0. 70? 0. 03 0. 50? 0. 01, t= 15. 014, P 0. 05, ,; 9 h 6 h, ,,FITC-conA , EPS, ; EPS, EPS ( 181. 19? 1. 59)L g (602. 66? 21. 94)L g, t= 60. 589, P 0. 05 PA01 EPS = ; ; ; = R378. 991 =A E ffects of allitride on adhesion and exopolysa ccha ride ofP seudom ona s a erug inosa b io- film s LIN Li-hua, YU Jia-lin, LIN Ya-yin, LIU Guan-xin (Ch ildrencsH osp ital of Chongqing M ed ical University, Chongqing 400014, China) =A bstrac t O bject ive To investigate the effectsof allitride on adhesion ofP seudom onas aerug inosa biofilm at early stage and Extracellular Polymeric Substances ( EPS). M ethods M ultifunction fluorometer was used to measure fluores- cence of green fluorescent protein(GFP) in pGFPuv transformated PA01 in 96-well plate. The adhesion ratio ofPA01was calculated to determ ine the effects of allitride on PA01biofilm adhesion, and qualitative study adherence ofGFP-PA01was performed by fluorescencemicroscope. The fluorescein isothiocyanate (FITC) conjugated concanavalinA ( FITC-conA) was used to stain the EPS, in order for the qualitation of EPS by fluorescence microscope; the pheno-l sulfuric acid method was used to quantitate the EPS in each group. R esu lts Six-hour group: after intervention with high dose of allitride, the adhe- sion ratio was decreased from 0. 70? 0. 03 to