改良腹腔多器官簇移植器官联合切取的实验研究.pdf

　2010 7 17 4　PrenterlEnterlNutrition, Vo.l17, No.4, July, 2010 ·247 · ·　 · 范　磊, 　李元新, 　郭明晓, 　王　凯, 　王　剑, 　黎介寿 , 210002 ( ) 　　[]　目的:(、、) 。 　方法:, , 0 ～4 ℃ (HCA) 。 　结果:(133.3 8 ±10.25 )min, , (19 8 ± 13.66 )min, (32.75 ±4.9 )min。　结论:, , 。 []　;　;　;　 ＊ []　R617　　[]　A　　[]　1007-810 X(2010)04-0247 -03 Experimentlstudyofhrvestingmethodsofmodifiedmultiviscerltrnsplnttion FANLe,iLIYun-xin, GUOMing-xio, WANGK,iWANGJin, LIJie-shou (ReserchInstituteofGenerlSurger,yCliniclSchoolofMediclCollegeofNnjingUniversity/Nnjing GenerlHospitlofNnjingMilitryCommnd, PLA, Nnjing210002, Jingsu, Chin) [Abstrct]　Objective:Toestblishmethodsofthegrfthrvestingndbck-tbleproceduresof modifiedmultiviscerltrnsplnttion(includingthestomch, duodenum, pncresndsm llintestine) in swinemode.l　Method:sFirs,tthemultiviscerlgrftwsdissected.Afterin-situlow-temperture perfusion, thegrftwsprocuredenblo.cFinlly, itwstrmimedndpreservedin0 ～4℃HCAfluid. Result:sThetmieofgrfthrvestingws(133.3 8 ±10.25 )minutes.Therewsnowrmischemitmie ndthecoldischemitmiews(198 ±13.66 )minutes.Thetmieofbck-tbleproceduresws(32.75 ± 4.95 )minute.s　Conclusion:Thisorgnhrvestingmethodneedsnowrmischemitmiendcnhelp hrvesthighqulitymultiviscerlgrf.t [Keywords]　Modifiedmultiviscerltrnsplnttion;　Multiviscerltrnsplnttion;　Grfthr- vestingmethod;　Swine 　　 (modifiedmultiviscerl 1　 trnsplnttion, MMVTx), 、 、, 1.1　实验动物　 23 ( 15 [1] 。, , 8)。3 ～6, 15 ～25 kg, ,。,