激素+内毒素-Gene Expression Profile of Steroid-induced Necrosis.pdfVIP

Calcif Tissue Int (2011) 89:271–284 DOI 10.1007/s00223-011-9516-y ORIGINAL RESEARCH Gene Expression Profile of Steroid-induced Necrosis of Femoral Head of Rats Peijian Tong • Chengliang Wu • Hongting Jin • Qiang Mao • Nanze Yu • Jonathan D. Holz • Letian Shan • Hui Liu • Luwei Xiao Received: 1 August 2010 / Accepted: 11 June 2011 / Published online: 22 July 2011 Springer Science+Business Media, LLC 2011 Abstract The key to treating steroid-induced necrosis of normal rats. A quantitative RT-PCR and immunohisto- femoral heads (SINFH) is early diagnosis. Dramatic chemistry (IHC) assays were used to confirm the micro- improvements in diagnosis could be made if the patho- array results. Compared to the control group, 190 genes in genesis of SINFH was more fully understood; however, the the experimental group were differentially expressed, with underlying mechanism of this disease is currently 52 up-regulated and 138 down-regulated. Of these genes, unknown. To explore the potential mechanism of SINFH, 102 are known (deposited in GenBank), while 88 of them we performed gene array analysis on a rat model of the are unknown. The known genes can be divided into several disease and compare the expression profile with that of families according to their biological functions, such as oxidative stress, apoptosis, signal transduction, angiogen- esis, extracellular matrix, lipid metabolism, and transcrip- The authors have stated that they have no conflict of interest. tion related genes. The results of quantitative RT-PCR and IHC were consistent wit