Reciprocal Effects of Guizhi Decoction (桂枝汤) to the Guizhi Decoction Syndrome by Toll-like Receptor mRNA Expression and Cytokines Secretion.pdfVIP

Reciprocal Effects of Guizhi Decoction (桂枝汤) to the Guizhi Decoction Syndrome by Toll-like Receptor mRNA Expression and Cytokines Secretion.pdf

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Reciprocal Effects of Guizhi Decoction (桂枝汤) to the Guizhi Decoction Syndrome by Toll-like Receptor mRNA Expression and Cytokines Secretion

·826· ORlGlNALARTICLE ReciprocalEffectsofGuizhiDecoction(桂枝汤)totheGuizhi DecoctionSyndromebyToll-likeReceptormRNA ExpressionaannddCCyyttOokkinnee=sSeeccrreettiioonn水 DUXin—liang(杜新亮),suIFeng(隋 峰),HUOHai-ru(霍海如),ZHANGHong-wei(张宏伟), LUOKan(罗 侃),LILan-fang(李兰芳)’,GUOShu-ying(郭淑英)’,andJIANGTing-liang(姜廷良)’ ABSTRACT Objective:ToexplorethepathologicalmechanismsofGuizhiDecoction(桂枝汤)syndromeandthe therapeuticmolecularmechanismsoftheGuizhiDecoction,MahuangDecoction(麻黄汤),SangjuDecoction(桑菊 饮)andYinqiaoPowder(银翘散),aswellasthepotentiallybiologicalbasisthatGuizhiDeocctionismosteffective onlyforthepatientswithGuizhiDecoctionsyndrome inclinicalpractice.Methods:W efirstgotserum samples frOm thepatientssufferingfrom bothupperrespiratorytractinfectionandGuizhiDecoctionsyndromeidentified bythedoctorsofChinesemedicine(CM)intheclinic.Fourformulaswiththerapeuticactionsofpungentwarmth orpungentcoolnessforsuperficialsyndromeswerechosenandfourkindsofratserum sampleseachcontaining oneoftheabove-mentionedherbalformulaswerecollected.thentheeffectsofGuizhiDecoctionsyndromes‘ patientserum aswellastheeffectsofseracontainingtheformulasafterbeingstimulatedbythepatientserum samplesonboththemRNAexpressionofcertaintoll-likereceptor(TLR)subtypesandthereleaseofsome inflammatorycytokinesinRAW 264.7cellsweretestedandanalyzedinvitro.Results:TheexpressionofTLR-3. TLR-4andTLR-9mRNAamongthe9testedTLR subformswereup-regulatedinthemacrophagesstimulated bytheserafrom untreatedupperrespiratoryinfectionpatientswiththeGuizhIDecoctionsyndromefsymptom · complex).Theproductssuchasinterleukin(IL)-113,IL-6,tumornecrosisfactor(TNF)- andinterferon(IFN)-B fn3m stimulatedmacrophagesthroughTLR signalingpathwayswerealsoincreasedcOrresp0ndingIy.Interestingly, thechangesinducedbytheGuizhiDecoctionsyndromepatients‘seraweremaskedsignificantlyafterthe macrophageswere incubatedwiththe

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