Genetic Diversity Analysis Among Populations of Mink from China by Inter-simple Sequence Repeat (ISSR) Markers.pdfVIP

Genetic Diversity Analysis Among Populations of Mink from China by Inter-simple Sequence Repeat (ISSR) Markers.pdf

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Genetic Diversity Analysis Among Populations of Mink from China by Inter-simple Sequence Repeat (ISSR) Markers

稚 scienceDireCt September2013 Vo1.20No.3 47—54 JournalofNortheastAgriculturalUniversity(EnglishEdition) AvailableonlineatvIvw.sciencediec1.tom GeneticDiversityAnalysisAmongPopulationsofMinkfrOm Chinaby Inter-simpleSequenceRepeat(ISSR)Markers YangHong—yan,FuJing,NingFang—yong,DuZhi—heng,andBaiXiu-juna’ CollegeofAnimalSciencesandTechnology,NortheastAgriculturalUniversity,Harbin150030,China Abstract:Inter—simpleSequenceRepeat(ISSR)analysiswasappliedtoassessthegeneticdiversitywithinandamongfive populationsofminkfrom LiaoningProvince.A totalof20primerswerescreened,fiveselectedprimersproduced35discemible bands,with30(85.71%)beingpolymorphic,indicatinghighgeneticdiversityatthespeciesleve1.Thehighestgeneticdiversiytwas observedinthebrownminkpopulation,whereasthelowestdiversiytwasfoundinthestandard-pitchyminkpopulation.Basedon geneticdistance(1972),adendrogramwasconstructedbyusingUPGMAalgorithm,andfivepopulationsweredividedintotwo majorgroups.GroupIconsistedofonlythestandard—pitchymink population,andGroupIIincludedohterfourpopulations,inGroup II,sapphiremink wasclosetobrownmink population.Theresultsofgeneticdifferentiationindicatedthatthegeneticdifferentiation dergeebewt eenpopulationswaslowernadhtegeneticvariationprimarilycamerfom withinpopulations.ThispapershowedhtatISSR techniquewasareliabletoolthatcouldbeusedtostudygeneticdiversityinthemink. Keywords:mink ,ISSR,geneticdiversiyt CLCnumber:Q953 Documentcode:A ArticlelD:1006—8104f2013)一03—0047-08 ISSRmarker(Inter—simpleSequenceRepeat lntroduction Markers)isaPeRbasedtechniqueusingprimers composedofmicrosatellitesequences(Zietkiewicz Mink(Mustelidae),asanimportnateconomicanimal, eta1.,1994).Itcombinestheadv

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